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| Molecular
Biology |
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| Genetic
Mapping |
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| Ecology |
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| Transgenics |
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| Bioinformatics |
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| Microscopy |
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| Paleontology |
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| Stickleback Molecular Genetics Course: |
A practical lab course, July 10th to 20th, 2011 at Stanford University. Optional stickleback fossil trip with Mike Bell, July 20th and 21st, 2011.
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| Sticklebacks |
Zebrafish |
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| Threespine stickleback fish offer a unique
opportunity to combine developmental, ecological, and paleontological
studies of vertebrate evolution. This summer course will offer hands-on
training in many new molecular techniques now being applied to this
system, as well as background in the biology, natural history, development,
and evolution of Gasterosteus aculeatus, zebrafish, and other vertebrates.
Resident and special guest faculty will interact extensively with
students in lecture, discussion, and lab sessions to introduce a variety
of field, experimental and computational methods that can be used
to study vertebrate evolution and development. Registration, room,
board and course materials will be provided by the Stanford Center
for Vertebrate Evolution (http://cegs.stanford.edu). |
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| Lab experiments
covering: |
- Field collecting and crossing sticklebacks by
artificial fertilization
- DNA microinjection of both zebrafish and stickleback
eggs
- Morpholino analysis to eliminate gene functions
and test effects on early embryonic development
- In situ hybridization analysis of gene expression
in developing fish
- Making DNA from fish, genotyping with microsatellite
markers
- Mapping genes and traits using genome-wide linkage
approaches
- Comparative genomics and bioinformatics
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| Lectures and discussions: |
- David Kingsley (Stanford): Genetics of
form and morphology in vertebrate systems. Genomic approaches
to identifying the genes and mutations responsible for evolutionary
divergence.
- Will Talbot (Stanford): Zebrafish developmental
genetics, gene duplication in vertebrate evolution.
- Katie Peichel (Fred Hutchinson Cancer Research
Center, Seattle): Sex determination, genetics of display and
courtship behavior in sticklebacks.
- Dolph Schluter (University of British Columbia.
Vancouver): Adaptive evolution in sticklebacks, formation
and divergence of benthic-limnetic species pairs
- Mike Bell (Stony Brook): Tempo of evolutionary
change in contemporary and fossil sticklebacks. Unique morphological,
behavioral, and life history traits in Alaskan fish populations.
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| Optional Paleontology
Field Trip after the course |
| Fossil history of stickleback evolution,
with sample collecting at a Miocene Lake quarry site located outside
Reno, Nevada (led by Mike Bell). After the course on July 20th and 21st. Transportation provided to site, and to Bay Area airports for departures in late afternoon and evening on July 21st |
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| Contact |
| Interested graduate students, postdocs,
or faculty members should send a CV and brief letter describing their
background, research interests, and how the course would fit into
their future career goals to: David Kingsley, Beckman Center B300,
279 Campus Drive, Stanford, CA 94305-5329, or send email to: kingsley@stanford.edu |
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